The purpose of this review is to explore the impact of mindfulness-based stress reduction (MBSR) on the brain. Neuroimaging studies using both clinical and nonclinical samples are reviewed herein, with a particular focus on functional and structural related brain changes associated with participation in MBSR. Despite a number of methodological limitations, these preliminary neuroimaging investigations revealed that MBSR training affects areas of the brain related to attention, introspection, and emotional processing consistent with the outcomes observed in the clinical literature. Directions for future research are discussed throughout. To further elucidate the processes through which mindfulness exerts its effects on the brain future studies using larger sample sizes, incorporating imaging tasks with performance measures, including longer-term follow-ups, and quantifying the relationship between home practice adherence with structural and functional changes are needed.