This study examined the cost of implementing the Cultivating Awareness and Resilience in Education (CARE) for Teachers professional development program during a randomized controlled trial targeting a diverse sample of public elementary school teachers in New York City. Detailed budget information collected during the study was used to identify the cost of all necessary resources associated with program implementation. The largest expense category was opportunity costs associated with teacher participation, accounting for over 40% of the total cost. This was closely followed by the program-required costs related to coordination, facilitation, and supplies for the program (31.8%). Finally, ancillary costs related to facilitator travel, room rental, and food for the program implementations encompassed 11% of the total cost. Across all three program implementations, 118 teachers were trained; the average cost per teacher was US$1217 when accounting for all categories. In future cost projections for a training with 30 teachers, the price per teacher is only US$515 when considering program-required and indirect costs. The costs for implementing the CARE for Teachers program are similar to those reported for other mindfulness-based interventions (MBIs). This paper provides a detailed analysis of the full cost of providing an evidence-based MBI to teachers in a public education setting. This research can help inform communities interested in funding future CARE for Teachers program implementations, provide an example for cost reporting for other MBIs, and provide a basis for future cost-effectiveness and benefit-cost analyses.